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The Value of Molecular Typing in the Assessment of Bacterial Translocation During Coagulase-Negative Staphylococcal Bacteremia in Preterm Infants

Andre Leke, Guy Kongolo, Mickael Frere-Moysan, Ghida Ghostine, Christele Chazal, Maurice Biendo

Background: One hundred fifty-seven preterm infants who were enrolled in the study were hospitalized between 2012 and 2014 at the University Hospital of Amiens-Picardie. Only 28 (17.8%) of the children who had a secondary coagulase-negative staphylococcal bacteremia with positive stool cultures were included in this study. Objectives: This investigation sought to examine the rate of intestinal bacterial translocation associated with these infections. Methods: In the context of this study, blood and stool cultures were performed. MALDI-TOF MS was used to examine all isolates of Staphylococcus spp. Genotyping and antibiotic susceptibility were also done. Results: Antibiotic susceptibility revealed sixteen resistance patterns in blood and stool. Ten of the coagulasenegative Staphylococcus strains isolated from blood samples showed an R pattern e (35.7%) and eleven of the coagulase-negative Staphylococcus strains isolated from stool samples showed an R pattern e (39.2%). 53.5% of cases in blood culture results were similar to stool culture results and in 46.5% of cases they were different. Fifteen different bacteria had three different patterns: ERIC-2 (A, B and C) and RAPD-PCR (D, E and F). ERIC-2 patterns comprised A (S. epidermidis isolates); B (S. haemolyticus isolates) and C (unidentified coagulasenegative Staphylococcus isolates). RAPD patterns consisted of D (unidentified coagulase-negative Staphylococcus isolates); E (S. haemolyticus isolates) and F (S. epidermidis isolates). Conclusion: Bacterial translocation from the gastrointestinal tract may have been the cause of coagulasenegative staphylococcal bacteremia in hospitalized preterm infants.