营养与食品科学杂志
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枸杞水溶性植物化学物质下调内质网应激生物标志物并调节多种信号通路，从而抑制 Jurkat 细胞增殖和诱导细胞凋亡

姜宇、张宇农、Logan Wark、Edlin Ortiz、Soyoung Lim、何慧、王伟群、Denis Medeiros 和 Dingbo Lin

Phytochemicals have received much recent attention in cancer prevention through simultaneous targeting multiple pathways in the disease progression. Here we determined that wolfberry phytochemicals was chemopreventive on the leukemic Jurkat cell. The water soluble wolfberry fractions (i.e., wolfberry phytochemicals) were enriched in carbohydrates (73.4 ± 4.5 % (w/w)), polyphenolics (1555 ± 112 mg quercetin equivalent / 100 g freeze dry powder, including 213 mg rutin/100 g freeze dry powder), and had enhanced antioxidant activity (7771 ±207 μM Trolox equivalent/ 100 g freeze dry powder). Wolfberry phytochemicals, but not purified wolfberry polysaccharide fractions, inhibited Jurkat cell proliferation, induced cycle arrest at the G2/M phase in a dose dependent manner starting at 1 mg/ml for 48 h. Wolfberry phytochemicals eliminated cellular reactive oxygen species, declined expression of endoplasmic
网状 (ER) 应激生物标志物，包括葡萄糖调节蛋白 78、肌醇需要蛋白 1 (IRE1)、
激活转录因子 6 (ATF6)、蛋白激酶 RNA 样 ER 激酶 (PERK) 和 c/EBP 同源蛋白，以及诱导 AMP 激活蛋白激酶的激活、β-连环蛋白的稳定以及 NFκB 和 AKT 活性的抑制。同时 siRNA 敲低 ATF6、IRE1 和 PERK 会抑制细胞增殖并诱导细胞凋亡。数据表明，在细胞凋亡进展过程中，内质网应激和多种存活/凋亡信号通路受到枸杞植物化学物质的调节。食用枸杞可能是预防白血病的有效饮食策略。