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Extraction and Isolation of Anthocyanin from Waste Hibiscus Flower using Column Chromatography and its Characterization

Ritika Saini, Ankur Rastogi, Mukesh Jangir*

Anthocyanin has attracted much attention for exhibiting phytochemical activities such as anti-inflammatory, antioxidant and anticancer properties and has blood pressure lowering and liver protective effects. Hibiscus rosa-sinensis contains phytochemical compound anthocyanin which has therapeutic uses and can be used for medicinal purpose the objective of this study is to utilize this waste flower and extract phytochemical compounds from it using acidified water followed by its isolation using column chromatography and its characterization by TLC and paper chromatography technique.