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生活方式因素和实验室精子处理技术与精子 DNA 碎片化指数、氧化应激加合物和高 DNA 染色性之间的相关性

艾丽西亚·莱尼亚·布罗萨德、本杰明·利德、埃德娜·蒂拉多、海伦娜·拉塞尔、欣德·贝杜恩、罗伯特·科尔弗、劳拉·路透、布拉德福德·波普、马修·威尔、艾丽卡·安斯帕赫·威尔、格伦·阿达尼亚

Purpose: To determine correlation between lifestyle risk factors and sperm quality.

Methods: Patients (n=133) who consented for the study completed a lifestyle questionnaire. An aliquot of sperm was frozen at three different time points. Preparation methods for 30 semen analysis were compared: ZyMōt Sperm Separation Device (DxNow), Isolate gradient (Irvine), SpermGrad gradient (Vitrolife), and each gradient was followed by swim-up (SU), Isolate+SU and Spermgrad+SU. All samples were analyzed using the Sperm DNA Fragmentation Assay (acridine orange/flow cytometry SDFA™). Analysis included DNA Fragmentation Index (DFI), Oxidative Stress Adducts (OSA) and High DNA Stainability (HDS). Statistical analysis was performed using JMP (SAS 2018) and P<0.05 was considered statistically significant.

Results: The neat DFI was not correlated with age, morphology, or oligospermia (<20 million/mL). Men that consumed alcohol daily trended towards a higher DFI than those that drank multiple times per week and significantly higher than those who never drink (p=0.0608 and p=0.0290, respectively), but interestingly not those who drank rarely. DFI was also positively correlated with OSA and HDS in the neat and processed sample (INSEM). The DFI of the INSEM sperm sample was positively correlated with age, poor morphology, and oligospermia (p=0.0208, p<0.0001, p=0.0006, respectively). There was no correlation with BMI or smoking status for neat or processed sperm health. The separation device effectively improved the DFI, OSA, and HDS compared to other methods

Conclusion: Lifestyle factors and preparation method is correlated with sperm quality.