蛋白质组学与生物信息学杂志
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大鼠脑匀浆放热过程的蛋白质组学分析

Y Voynikov、L Velkova、L Tancheva、P Mladenov、A Dolashki、L Alova、W Voelter 和 P Dolashka

Alzheimer’s disease (AD) is the most widespread neurodegenerative disorder which can be induced by scopolamine, but the underlying molecular mechanism is poorly understood. Recently, differential scanning calorimetry (DSC) has been used to study healthy and scopolamine-treated mice. A well-expressed exothermic transition minimum in the range of 35 - 45°C was determined in the DSC profiles of healthy mice supernatants. To explain this process, using two-dimensional gel electrophoresis (2D-PAGE) coupled with MALDI-TOF-TOF, poorly soluble membrane proteins in hippocampal proteome of rat brain tissue were identified. The different behavior of the hippocampal proteome from the healthy rats before and after heating to 45oC was identified. Due to the demonstrated change in protein level of tau protein and tubulin in the rat hippocampus after heating to 45°C, it was suggested that the observed exothermic process at 35-45°C in rat may be due to the partial unfolding of tau protein, which leads to the release of tubulin. Both proteins together are involved in protein fibrillation and aggregation.

另一个重要结果是发现了东莨菪碱诱导的神经退行性疾病大鼠海马匀浆的蛋白质组差异及其与健康大鼠的特征。

这项研究报告的结果有助于阐明东莨菪碱诱导的痴呆和神经退行性过程的分子机制。