国际标准期刊号: 2471-9315
Ping-An Wang
Ganoderma lucidum, a kind of basidiomycete, can produce various valuable metabolites with bioactive. For a long time, the shortage of the genome and gene manipulation methods limited the analysis of gene functions, which delayed the elucidation of biosynthesis pathways of those products in G. lucidum. As G. lucidum was sequenced and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) system was established, gene modification and gene function analysis was feasible. In this review, we summarized the establishment of CRISPR/Cas9 system and its biotechnological application in G. lucidum. Besides, we discuss challenges to the current CRISPR/Cas9 system and give perspectives on the biotechnology research in G. lucidum.