郑健1、李晓曦1、江友红2、蔡存伟3
目的：探讨胸腺素是否影响肿瘤细胞表面PD-L1的表达，进而影响三阴性乳腺癌的免疫治疗。
方法：用不同浓度的胸腺肽α1处理人乳腺癌MDA-MB-231细胞后，检测细胞PD-L1蛋白和基因的表达。通过生物信息学分析，筛选出目的基因，进一步验证了胸腺肽α1可能参与的信号通路。
R After adding 130 μM and 160 μM of thymosin α1, the expression of PD-L1 in MDA-MB-231 cells was inhibited at 12 h, 24 h, 48 h and 96 h. The related pathways of genes down-regulated by Tα1 in KEGG are: Cancer pathway, basal cell epithelioma, apoptosis, Hippo and Wnt signaling pathway; immune pathway, such as rheumatoid arthritis, bacterial infection. After MSAB treatment of the experimental group, the expression of AXIN2 was up-regulated, and the expression of β-catenin in the cells was up-regulated. Compared with the cells in the untreated experimental group, PD-L1 was up-regulated. However, we found that β-catenin in the nucleus was not significantly up-regulated, but the expression of C-myc was up-regulated result.
结论：胸腺素α1通过Wnt/β-catenin通路下调TNBC的PD-L1表达，可能影响PD-L1依赖性TNBC免疫治疗。