植物生物化学与生理学杂志
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Sa和Meja处理白桦幼树生理特性、氧化角鲨烯环化酶基因表达及三萜类化合物积累

殷静、梁田、张梦艳、李春晓、肖家雷、詹亚光

桦树（Betula platyphylla suk）中的五环三萜类化合物具有广泛的药理活性，可用于开发抗癌和抗艾滋病药物。在本研究中，我们探讨了喷洒不同浓度的茉莉酸甲酯（MeJA）和水杨酸的3年生白桦的效果。(SA)对桦树幼苗三萜类化合物生物合成途径关键基因表达及三萜类化合物积累及生理特性的影响。结果表明，喷施不同浓度的MeJA和SA均能明显促进3年生白桦总三萜类化合物的积累。1 mmol·L-1 MeJA（MJ2）处理1 d后茎皮中三萜含量显着增加，5 mmol·L-1 SA（SA1）处理14 d后，MJ2和SA1的三萜含量均达到81.86 mg/g和91.40 mg/g,分别比对照(CK)增加了46.11%和45.07%。此外，MeJA和SA处理增加了叶绿素a和b、抗氧化酶超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）的含量，以及光合酶的含量。 performance, and affected the content of soluble sugar and soluble protein in birch leaf. Fluorescence quantitative polymerase chain reaction (qPCR) results showed that MeJA and SA treatment deferentially enhanced the expression of key genes farnesyl diphosphate synthase (FPS), cycloartenol synthase
(BPX and BPX2), lupeol synthase (BPW) and beta-amyrin synthase (BPY) in triterpenoid synthesis pathway in birch bark and leaves. The results showed that MeJA and SA induced triterpenoid synthesis of birch plant is closely related with not only the expression of key genes of triterpenoid synthesis pathway but also photosynthesis, anti-stress response and physiological indexes, suggesting regulation of triterpenoid synthesis of birch by MeJA and SA may involve in more complex mechanisms at physiological and molecular level.