临床毒理学杂志
开放获取

抽象的

Evaluation Acute Toxicity Studies of Hydro Alcoholic Extract of Soursop Leaves (Annona muricata) on Brain, Liver and Kidney of Wister Rats

Shalu Chauhan*, Vivek Srivastava

Objectives: The present study was undertaken to evaluate the acute oral toxicity of hydro-alcoholic leave extract of Annona muricata and to derive the LD₅₀. The purpose of the study was to investigate acute toxicity test of soursop leave extract on Wister rat brain, liver and kidney.

Methods: Acute toxicity studies of hydro alcoholic leave extract of Annona muricata were conducted as per the OECD guidelines 407. The animals were grouped into three test and one control groups. Except for treatment with the test substance, animals in the control group were Liaw led in an identical manner to the test group subjects. The mice were observed until day 7 to determine the LD₅₀ and at the end were terminated to collect the brain, liver and kidney. The organs later were made into histopathology slides. The slides read with light microscope. Data were assessed by one-way ANOVA followed by Tukey’s multiple comparison tests. Values for which starting from p<0.05 was considered as statistically significant.

Results: The acute toxicity study done using OECD 420 guideline revealed that hydro alcoholic leave extract of Annona muricata were safe at 100 mg/kg and produced mortality at 1000 and 2500 mg/kg. On gross examination, body weight, biochemical parameter (Serum Glutamic-Oxaloacetic Transaminase (SGOT), Serum Glutamic Pyruvic Transaminase (SGPT), blood urea) was observed.

Conclusion: The present study concluded the toxicity produced by A. muricata leave in the biochemical parameter and organ (brain, liver and kidney). The LD₅₀ of soursop leave extract is more than 2500 mg/kg BW. This result indicate that the low dose of extract is practically nontoxic and do not damage the brain, liver and kidney.